L1 /
14
CH3041
HPLC IV
• A good quality pump is used to reduce
noise. Columns (expensive) are protected by a short guard column to prevent rubbish from degrading the main analytical column.
noise. Columns (expensive) are protected by a short guard column to prevent rubbish from degrading the main analytical column.
Detectors:
• RI Refractive Index
: universal detector, as
all solutes have a RI slightly different to the
MP. Cannot use gradients & low sensitivity.
all solutes have a RI slightly different to the
MP. Cannot use gradients & low sensitivity.
• UV-Vis
: for cmpds with a chromophore.
PhotoDiode
Array : all wavelengths can be
measured in a single instant, allowing 3 scans per band to verify band purity.
measured in a single instant, allowing 3 scans per band to verify band purity.
• MS
: provides details on MW and structure,
expensive as it requires a sophisticated
vacuum system. - LCMS
expensive as it requires a sophisticated
vacuum system. - LCMS
