L1 /   
5
CH3041
CHROMATOGRAPHY THEORY
 A mobile D A stationary
Partition coefficient: K = [Astation] / [Amobile]
Column performance
1) Number of plates:  N = 16 (tr / W)2 (vs time)
2) Peak asymmetry:
Loading: loadlarge/loadsmall = (radiuslarge/radiussmall)2
Gas Chromatography
Mobile Phase = He, flow rate 1mL/min.
                                                          Gaussian             asymmetric
Relative retention: a = K2/K1 = tr2’/tr1’
 this is the physical basis for chromatography
Resolution:            2(tr2 - tr1) / (W2 + W1)