SEPARATION 1) If there is a mixture
of analytes then a separation
is often needed to resolve all of them in the analysis.
2) If interfering
compounds are present
then these must be separated out from the analyte. If both the matrix & analyte are well characterised
then interfering cmpds may be
predicted in advance. Otherwise trial analyses must be run, any interferences identified
and a separation protocol developed.
eg. pesticides in cane, must remove sugar
to identify trace amounts of a pesticide.