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CH1012
UV SPECTROSCOPY II
Among organic compounds only those which are unsaturated & particularly those which are conjugated will absorb radiation (UV) in this way.
Beer-Lambert
Law
(Beer’s Law)
(Beer’s Law)
e molar absorption
coefficient
A = e c l
coefficient
A = e c l
A = absorbance
c = conc. (M)
l = path length (cm)
UV Spectroscopy = “conjugation detector +C=O”
lmax
